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The growth characteristics of ΔcaiA , mirroring those of the parental strain BW25113 in both liquid culture and on agar plates, indicate that the observed embryonic lethality stems not from restricted bacterial growth or nutritional scarcity but from the specific absence of L-carnitine synthesis capability in both the worm mutant and ΔcaiA

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All P values were two-tailed, and those 0.1) (Figure S3)