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In brief, lung tissue was homogenized in 0.5 ml of 0.5 % hexadecyltrimethyl ammonium bromide in 50 mM potassium phosphate buffer (pH 6.0)

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Choline supplementation of the mothers of unborn rats, as well as rat pups during the first month of life, leads to improved performance in spatial memory tests months after choline supplementation has been discontinued (123)